Aim: To study the genome profile
of Chital deer (A. axis) by microsatellite markers using faecal DNA & to
assess its usefulness as non invasive method of genotyping.
Materials and
Methods: DNA was isolated from 44 animals from faeces and blood, out of
which 26 (3 from blood and 23 from faeces) were subjected for genotyping. The
PCR products of all the ten microsatellite loci were subjected to 6 % Urea- PAGE
to differentiate allelic size.
Result: Total 57 alleles and 49 genotypes
were found with their frequencies ranging from 0.022 to 0.457 and 0.043 to 0.696
respectively. The observed number of alleles ranged from 4 -8, (Av. = 5.7 per
locus). The PIC and Shannon's information index values for all loci ranged
between 0.6645 to 0.8270 (Av. = 0.7442 per locus) and 1.1870 to 1.8852 (Av. =
1.5122 per locus) respectively.
Conclusion: Faecal DNA can be effectively
used in genotyping wild animals, however the method and time of collection &
storage as well as the type of faecal material largely influences the quality &
quantity of DNA. Keywords: chital deer, faecal DNA, microsatellites, PIC,
urea – PAGE
